Phospho-CD130/gp130 (Ser782) Rabbit Polyclonal Antibody

• Catalog Number: orb704492
• Category: Antibodies
• Description: Phospho-CD130/gp130 (Ser782) Rabbit Polyclonal Antibody
• Target: IL6ST
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rabbit, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human gp130 around the phosphorylation site of Ser 782 SE(p-S)TQ
• UniProt ID: P40189
• MW: 99 kDa
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: gp130(phospho S782); gp130(phospho Ser782); p-gp13
• Note: For research use only

Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Liver (Rat) Lysate at 40 ug, Lane 3: Lung (Rat) Lysate at 40 ug, Primary: Anti-phospho-CD130/gp130 (Ser782) (orb704492) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 140 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-gp130 (Ser782) Polyclonal Antibody, Unconjugated (orb704492) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.