Phospho-CD130/gp130 (Ser782) Rabbit Polyclonal Antibody

• Catalog Number: orb157259
• Category: Antibodies
• Description: Phospho-CD130/gp130 (Ser782) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine
• Reactivity: Human, Rabbit, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human gp130 around the phosphorylation site of Ser782 SE(p-S)TQ
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IL6ST
• UniProt ID: P40189
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: gp130(phospho S782); gp130(phospho Ser782); p-gp13
• Background: _CLIENT_OVERRIDE_
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (orb157259) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (orb157259) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

phosphopeptide, non phosphopeptide.

Sample: Lane 1: Human HepG2 cell Lysates, Lane 2: Human HUVEC cell Lysates, Primary: Anti-phospho-CD130gp130 (Ser782) (orb157259) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130kDa, Observed band size: 130kDa.