PGP9.5 / UchL1 Antibody

• Catalog Number: orb750274
• Category: Antibodies
• Description: This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, UchL1 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for UchL1 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in the gene is believed to cause a form of Parkinson's disease.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Native UchL1/PGP9.5 protein isolated from human brain was used as the immunogen for this anti-UchL1 antibody.
• UniProt ID: P09936
• Tested applications: WB
• Dilution range: Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: SPM575
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This anti-UchL1 antibody is available for research use only.
• Note: For research use only

Western blot testing of human brain lysate with anti-UchL1 antibody (clone SPM575). Predicted molecular weight ~25 kDa.

SDS-PAGE analysis of purified, BSA-free anti-UchL1 antibody (clone SPM575) as confirmation of integrity and purity.