PGP9.5 / UchL1 Antibody

• Catalog Number: orb750152
• Category: Antibodies
• Description: UCHL1/PGP9.5 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of PGP9.5 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is abundantly present in all neurons (accounts for 1-2% of total brain protein), expressed specifically in neurons and testis/ovary. [Wiki]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: UBCE-L1
• Tested applications: IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the PGP9.5 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This PGP9.5 antibody is available for research use only.
• UniProt ID: P09936
• Storage: Store the PGP9.5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PGP9.5 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE rat cerebellum with PGP9.5 antibody (clone UBCE-L1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human brain lysate with PGP9.5 antibody (clone UBCE-L1). Predicted molecular weight ~25 kDa.