PGP9.5 / UchL1 Antibody

• Catalog Number: orb749836
• Category: Antibodies
• Description: This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Immunogen: Recombinant human protein was used as the immunogen for the PGP 9.5 antibody.
• UniProt ID: P09936
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the SUMO-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: UCHL1/775
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This PGP 9.5 antibody is available for research use only.
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded rat testis stained with PGP 9.5 antibody (UCHL1/775).

IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with PGP 9.5 antibody (UCHL1/775).

Western blot testing of human brain lysate with PGP 9.5 antibody (clone UCHL1/775). Predicted molecular weight ~25 kDa.

Western blot testing of human brain lysate with PGP 9.5 antibody (clone UCHL1/775). Predicted molecular weight ~25 kDa.