PGP Antibody

• Catalog Number: orb1520653
• Category: Antibodies
• Description: PGP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 271 and 321
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PGP
• UniProt ID: A6NDG6
• NCBI: A6NDG6.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: AUM; Phosphoglycolate phosphatase; PGPase; PGP; as
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PGP by western blot. Samples: Whole cell lysate (10 µg) from HeLa, MCF-7, HEK293T, Hep-G2, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PGP antibody (orb1520653) used for WB at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse PGP by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PGP antibody (orb1520653) used for WB at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human PGP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PGP antibody (orb1520653) used for IP at 6 µg per reaction.