CD44 Antibody

• Catalog Number: orb402179
• Category: Antibodies
• Description: CD44 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human CD44 recombinant protein (Position: Q21-H259).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 82 kDa
• UniProt ID: P16070
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: CD44 antigen; CDw44; Epican; Extracellular matrix
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of Jurkat cells using anti-CD44 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD44 using anti-CD44 antibody.Lane 1:human HeLa Cell;2:human 22RV1 Cell;3:rat spleen Tissue;4:rat thymus Tissue;5:mouse spleen Tissue.

IF analysis of CD44 using anti-CD44 antibody.CD44 was detected in paraffin-embedded section of mouse lymphaden tissues.

IF analysis of CD44 using anti-CD44 antibody.CD44 was detected in paraffin-embedded section of mouse lymphaden tissues.

IHC analysis of CD44 using anti-CD44 antibody.CD44 was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of CD44 using anti-CD44 antibody.CD44 was detected in paraffin-embedded section of human placenta tissue.

IHC analysis of CD44 using anti-CD44 antibody.CD44 was detected in paraffin-embedded section of rat kidney tissue.

IHC analysis of CD44 using anti-CD44 antibody.CD44 was detected in paraffin-embedded section of mouse kidney tissue.