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Catalog Number | orb1958306 |
---|---|
Category | Proteins |
Description | PEDF Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.8 kDa and the accession number is A0A140VKF3. |
Tag | C-His |
Purity | 98.00% |
UniProt ID | P36955 |
MW | 45.8 kDa (predicted); 45.8 kDa (reducing conditions) |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | PEDF Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.8 kDa and the accession number is A0A140VKF3. |
Expression Region | A DNA sequence encoding the human SERPINF1 (NP_002606.3) (Met 1-Pro 418) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 20 |
Storage | -20°C |
Note | For research use only |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.2 kDa after removal of the signal peptide. The apparent molecular mass of PEDF-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by SDS-PAGE | |
55 kDa |
Greater than 90% as determined by SDS-PAGE. | |
Escherichia Coli |