PECAM1 Antibody

• Catalog Number: orb1274245
• Category: Antibodies
• Description: PECAM1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: C31/1395R
• Tested applications: FC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG, k
• Immunogen: Human full length recombinant protein was used as the immunogen for the recombinant CD31 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Immunofluorescence: 0.5-1 ug/mlFlow Cytometry: 0.5-1ug/10e6 cells in 0.1mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RTPrediluted IHC only format: incubate for 30 min at RT (1)Titering of the recombinant CD31 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PECAM1
• UniProt ID: P16284
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Platelet endothelial cell adhesion molecule, PECAM
• Note: For research use only
• Application notes: Immunofluorescence: 0.5-1 ug/mlFlow Cytometry: 0.5-1ug/10e6 cells in 0.1mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RTPrediluted IHC only format: incubate for 30 min at RT (1)Titering of the recombinant CD31 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: steam sections in pH7.5-8.5, 1mM EDTA for 10-20 min.