PECAM1 Antibody

• Catalog Number: orb1252720
• Category: Antibodies
• Description: PECAM1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C31.7
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Primate, Rabbit
• Isotype: IgG1, kappa
• Immunogen: Human recombinant PECAM-1 / CD31 protein was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PECAM-1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PECAM1
• UniProt ID: P16284
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Platelet endothelial cell adhesion molecule, PECAM
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PECAM-1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of colon carcinoma with PECAM-1 antibody (C31.7). Note cell membrane staining of endothelial cells.

IHC staining of angiosarcoma with PECAM-1 antibody (C31.7).

IHC staining of tonsil tissue with PECAM-1 antibody (C31.7).

Western blot testing of human samples using PECAM-1 antibody (C31.7).

FACS testing of Jurkat cells: Black = cells alone; Green = isotype control; Red = PECAM-1 antibody PE conjugate.

SDS-PAGE Analysis of Purified, BSA-Free PECAM-1 Antibody (clone C31.7). Confirmation of Integrity and Purity of the Antibody.