PECAM1 Antibody

• Catalog Number: orb1252132
• Category: Antibodies
• Description: PECAM1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCM25-1
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, k
• Immunogen: Recombinant human protein was used as the immunogen for this CD31 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the CD31 antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PECAM1
• UniProt ID: P16284
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Platelet endothelial cell adhesion molecule, PECAM
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the CD31 antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

FACS testing of Jurkat cells with an isotype control (green) and CD31 antibody (red).

IHC testing of colon carcinoma and CD31 antibody (PCM25-1).

Western blot testing of ThP1 cells using CD31 antibody (PCM25-1).