PDIA3 antibody

• Catalog Number: orb18810
• Category: Antibodies
• Description: Goat polyclonal antibody to PDIA3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Dilution range: ELISA: 1:4000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 56.8
• Target: PDIA3 / ERp57 / GRP58
• Entrez: 2923
• Protein Sequence: EEKPKKKKKAQEDL
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PDIA3 antibody, anti GRP58 antibody, anti ERp
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 55kDa band observed in human hepatoblastoma HepG2 lysates (calculated MW of 56.8kDa according to NP_005304.3). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18810 staining (1µg/ml) of HepG2 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.