PDCD1 Antibody

• Catalog Number: orb1252135
• Category: Antibodies
• Description: PDCD1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PD203-1
• Tested applications: FC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the anti-PD-1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Immunofluorescence: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlTitering of the anti-PD-1 antibody may be required for optimal performance.1. FFPE testing requires tissue to be boiled in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PDCD1
• UniProt ID: Q15116
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD
• Note: For research use only
• Application notes: Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Immunofluorescence: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlTitering of the anti-PD-1 antibody may be required for optimal performance.1. FFPE testing requires tissue to be boiled in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil stained with anti-PD-1 antibody (clone PD203-1).