PDCD1 Antibody

• Catalog Number: orb1238787
• Category: Antibodies
• Description: PDCD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: PD-1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human PD-1.The immunogen is located within amino acids 120 - 170 of PD-1.
• Concentration: 1 mg/mL
• Dilution range: PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 32 kDa Observed: 43 kDa
• Target: PDCD1
• UniProt ID: Q15116
• NCBI: Q15116
• Storage: PD-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD
• Note: For research use only
• Application notes: PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 µg/mL in the (A) absence and (B) presence of blocking peptide.

Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 5 µg/mL.

Immunofluorescence of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 20 µg/ml. Green: PD-1 Antibody (orb1238787) Blue: DAPI staining.

Immunocytochemistry of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 10 µg/ml.