PD-L1/CD274 Antibody

• Catalog Number: orb215908
• Category: Antibodies
• Description: PD-L1/CD274 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 33275 MW
• UniProt ID: Q9NZQ7
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Programmed cell death 1 ligand 1;PD-L1;PDCD1 ligan
• Note: For research use only
• Application notes: WB: The detection limit for PD-L1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of JK cells using anti-PD-L1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of PD-L1 using anti-PD-L1 antibody.Lane 1:human PC-3 cell; 2:human placenta tissue; 3:human HepG2 cell; 4:human THP-1 cell; 5:human K562 cell; 6:human A549 cell; 7:human HeLa cell.