PCNA antibody

• Catalog Number: orb43327
• Category: Antibodies
• Description: Goat polyclonal to PCNA.Reported variants represent identical protein: NP_872590.1, NP_002583.1 .
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Concentration:

  0.5 mg/ml

• Dilution range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 28.8
• Target: PCNA (aa111-122)
• Entrez: 5111
• Protein Sequence: EAPNQEKVSDYE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti cyclin antibody, DNA polymerase delta auxilia
• Note: For research use only
• Application notes: WB: Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8kDa according to Human NP_002583.1). Approx.35kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33kDa in Pig Spleen lysates. (calcu. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 2.5µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Jurkat lysate (35ug protein in RIPA buffer) using PCNA antibody

Western blot analysis of NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35ug protein in RIPA buffer) using PCNA antibody

Western blot analysis of Pig Spleen lysate using PCNA antibody

Immunohistochemical staining of Human Tonsil using PCNA antibody.