PCNA antibody

• Catalog Number: orb402808
• Category: Antibodies
• Description: Mouse monoclonal antibody to PCNA
• Clonality: Monoclonal
• Clone Number: PC10
• Tested applications: FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Drosophila, Gallus, Human, Mouse, Primate, Rat
• Isotype: Mouse IgG2a kappa
• Immunogen: recombinant rat PCNA
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: PCNA
• Entrez: 5111
• UniProt ID: P12004
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-ATLD2 antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of K562 cells stained using anti-PCNA (PC10) purified antibody (concentration in sample 4 µg/ml, GAM FITC, red-filled) from K562 cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining).

Immunocytochemistry staining of PCNA in PHA-stimulated lymphocytes using mouse monoclonal antibody PC10 (diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200); DNA stained with propidium iodide. Colocalization of green PCNA signal and red DNA signal indicated as yellow.

Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-PCNA (PC10), 10 µg/ml.