PCNA antibody

• Catalog Number: orb214367
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PCNA
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PCNA
• Entrez: 25737, 5111, 18538
• UniProt ID: P04961, P17918, P12004
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Proliferating cell nuclear antigen antibody,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhou, Xiaohu et al. Metformin Inhibit Lung Cancer Cell Growth and Invasion in Vitro as Well as Tumor Formation in Vivo Partially by Activating PP2A Med. Sci. Monit., 25, 836-846 (2019)

PubMed: 30693913

Western blot analysis of PCNA expression in HEK293T (A), mouse testis (B), rat testis (C) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 36 kD)

Immunofluorescent analysis of PCNA staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.