PAX2 Antibody

• Catalog Number: orb1151874
• Category: Antibodies
• Description: Mouse monoclonal antibody to PAX2
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PAX2/1105
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, lambda
• Immunogen: A portion of amino acids 223-354 was used as the immunogen for the PAX2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA (For coating order BSA-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This PAX2 antibody is available for research use only.
• UniProt ID: Q02962
• Storage: Aliquot the PAX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PAX2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon tissue with PAX2 antibody (clone PAX2/1105). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot analysis of (1) recombinant PAX2 protein fragment and (2) human kidney lysate using PAX2 antibody (clone PAX2/1105). Expected molecular weight ~42 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using PAX2 antibody (clone PAX2/1105). These results demonstrate the foremost specificity of the PAX2/1105 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.