PAX2 Antibody

• Catalog Number: orb1151874
• Category: Antibodies
• Description: Mouse monoclonal antibody to PAX2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, lambda
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 223-354 was used as the immunogen for the PAX2 antibody.
• UniProt ID: Q02962
• Tested applications: ELISA, IHC-P, WB
• Dilution range: ELISA (For coating order BSA-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the PAX2 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PAX2/1105
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This PAX2 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human colon tissue with PAX2 antibody (clone PAX2/1105). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot analysis of (1) recombinant PAX2 protein fragment and (2) human kidney lysate using PAX2 antibody (clone PAX2/1105). Expected molecular weight ~42 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using PAX2 antibody (clone PAX2/1105). These results demonstrate the foremost specificity of the PAX2/1105 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.