PARP1 antibody

• Catalog Number: orb535110
• Category: Antibodies
• Description: PARP1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-PARP1 (N-term ZF1) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with n-terminus region of human PARP1 zinc finger domain recombinant protein. Anti-Rabbit IgG HRP secondary antibody was produced by repeated immunizations in goat with Rabbit IgG whole molecule.
• Form/Appearance: Liquid (sterile filtered)
• Purity: This PARP1 Antibody Set contains: PARP1 (N-term ZF1) protein, Rabbit Anti-PARP1 Antibody, and Goat Anti-RABBIT IgG (HRP) Antibody. PARP1 (N-term ZF1) protein is an N-terminus His-Tag recombinant protein expressed in E.coli corresponding to a fragment of the human PARP1 zinc finger domain. Rabbit Anti-PARP1 (N-term ZF1) was purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human PARP1 protein. No cross reactivity detected towards other PARP members when using siRNAs against 18 PARP family members. Cross-reactivity with PARP1 from other sources has not been determined. Goat Anti-RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
• Conjugation: Unconjugated
• UniProt ID: P09874
• Storage: Control Protein and Primary antibody: the vial contains a relatively low volume of reagent (25 µL). Store vial at -20° C or below prior to opening. To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Secondary antibody: Store vial at -20° C. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide and 0.01% (w/v) Gentamicin Sulfate
• Alternative names: Poly [ADP-ribose] polymerase 1, ADP-ribosyltransfe
• Note: For research use only
• Application notes: Anti-PARP1 (N-term ZF1) antibody with the matched secondary has been validated by western blotting and nanoimmunoassay (NIA). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 113 kDa in size corresponding to PARP-1 by western blotting in the appropriate cell lysate or extract. PARP1 (N-term ZF1) Control Protein analysis by SDS-PAGE resulted in a band at a MW ~13 kDa and estimated to be greater than 95% pure by Coomassie staining.
• Expiration Date: 12 months from date of receipt.