PARP/PARP1 Antibody

• Catalog Number: orb234355
• Category: Antibodies
• Description: PARP/PARP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PARP recombinant protein (Position: Q670-R858). Human PARP shares 94% and 95% amino acid (aa) sequence identity with mouse and rat PARP, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, RatImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatImmunocytochemistry , 2μg/ml, Human, RatImmunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 113084 MW
• UniProt ID: P09874
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Poly [ADP-ribose] polymerase 1;PARP-1;2.4.2.30;ADP
• Note: For research use only
• Application notes: WB: The detection limit for PARP is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of PARP using anti-PARP antibody.Lane 1:human HeLa cell;2:human HepG2 cell; 3:human COLO-320 cell; 4:human Jurkat cell;5:rat PC-12 cell;6:mouse NIH3T3 cell;7:mouse HEPA1-6 cell.

IHC analysis of PARP using anti-PARP antibody.PARP was detected in paraffin-embedded section of Mouse Intestine Tissue.

IHC analysis of PARP using anti-PARP antibody.PARP was detected in paraffin-embedded section of Rat Brain Tissue.

IHC analysis of PARP using anti-PARP antibody.PARP was detected in paraffin-embedded section of Human Placenta Tissue.

IHC analysis of PARP using anti-PARP antibody.PARP was detected in immunocytochemical section of A549 Cell.