PARP1 Antibody

• Catalog Number: orb1274565
• Category: Antibodies
• Description: PARP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2).
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:3000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200CHIP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 100kDa, 110kDa, 125kDa
• Target: PARP1
• UniProt ID: P09874
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: KIF3B, PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, A
• Note: For research use only
• Application notes: WB: 1:1000 - 1:3000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200CHIP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:500 dilution.

Western blot analysis of extracts of Jurkat cells, using PARP1 antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and PARP1 knockout (KO) HeLa cells, using PARP1 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using PARP1 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using PARP1 antibody at dilution of 1:200 (40x lens).