PARP1 Antibody

• Catalog Number: orb1294350
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PARP 1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide corresponding to residues surrounding Gly623 of human PARP. This antibody does not cross-react with PARP-2 and PARP-3.
• Dilution range: Western Blot 1:1000-1:1500 Immunofluorescence 1:100-1:300 Immunohistochemistry (Paraffin) 1: 100-1:150
• Conjugation: Unconjugated
• Target: PARP1
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PARP1 antibody - 1/1000 dilution. Lysates at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with orb1294350 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PARP1 cleaved with orb1294350 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PARP1 cleaved with orb1294350 at 1/200.

Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294350 Anti-PARP cleaved antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.