Ornithine Decarboxylase Antibody / ODC1

• Catalog Number: orb2637871
• Category: Antibodies
• Description: This antibody recognizes a 53kDa protein, identified as the Ornithine Decarboxylase. ODC-1 is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC-1 mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. Activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ODC1/485
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human ODC-1 protein was used as the immunogen for this antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1) (2)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This ODC-1 antibody is available for research use only.
• Entrez: 4953
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin/paraffin human prostate carcinoma stained with ODC-1 antibody (clone ODC1/485).

Western blot testing of human placenta lysate using ODC-1 antibody (clone ODC1/485).

Flow analysis of permeablized PC3 cells using ODC-1 antibody (clone ODC1/485).

Immunofluorescence testing of LNCaP cells and Alexa Fluor 488 conjugated ODC-1 antibody (clone ODC1/485).

Formalin/paraffin rat pancreas stained with ODC-1 antibody (clone ODC1/485).

Formalin/paraffin human testicular carcinoma stained with ODC-1 antibody (clone ODC1/485).

Formalin/paraffin mouse pancreas stained with ODC-1 antibody (clone ODC1/485).

SDS-PAGE analysis of purified, BSA-free ODC-1 antibody (clone ODC1/485) as confirmation of integrity and purity.

Western blot testing of human PC3 cell lysate using ODC-1 antibody (clone ODC1/485).