OCT-2 Antibody / POU2F2

• Catalog Number: orb2641325
• Category: Antibodies
• Description: Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. [UniProt]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity
• Immunogen: A portion of amino acids 112-297 from the human protein was used as the immunogen for the OCT-2 antibody.
• UniProt ID: P09086
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: The stated application concentrations are suggested starting points. Titration of the OCT-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Antibody Type: Primary Antibody
• Clone Number: PD2F2-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This OCT-2 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human tonsil with OCT-2 antibody (clone PD2F2-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human Ramos lysate with OCT-2 antibody (clone PD2F2-1). Expected molecular weight: isoforms from 43~62 kDa.