OCLN Antibody

• Catalog Number: orb1258098
• Category: Antibodies
• Description: OCLN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 263-522 of human Occludin (NP_002529.1).
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 59kDa
• Target: OCLN
• UniProt ID: Q16625
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Occludin, OCLN
• Note: For research use only
• Application notes: WB: 1:1000 - 1:2000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Occludin antibody (orb1258098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat pancreas using Occludin antibody (orb1258098) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using Occludin antibody (orb1258098) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Occludin antibody (orb1258098) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Occludin antibody (orb1258098) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using Occludin antibody (orb1258098) at dilution of 1:100. Blue: DAPI for nuclear staining.