You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1294379 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Occludin |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / Intracellular domain corresponding to Human Occludin |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200–1:400 Immunohistochemistry (Frozen section)1:100 |
Conjugation | Unconjugated |
Target | OCLN / occludin |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-Occludin antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294379 overnight at 4°C.
Occludin antibody by Frozen-section; Sample: Rat brain. Green: Occludin antibody diluted at 1:100. Blue: DAPI.
All lanes: Anti-Occludin antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294379 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294379 anti-Occludin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
ELISA, IF, IHC, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, IHC-P, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
Filter by Rating