Nucleolin Antibody

• Catalog Number: orb749713
• Category: Antibodies
• Description: Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human protein was used as the immunogen for the anti-Nucleolin antibody.
• UniProt ID: P19338
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Nucleolin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: SPM614
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This anti-Nucleolin antibody is available for research use only.
• Note: For research use only

Immunofluorescent staining of PFA-fixed human HeLa cells with anti-Nucleolin antibody (clone SPM614, green) and DAPI (blue).

Immunofluorescent staining of PFA-fixed human K562 cells with anti-Nucleolin antibody (clone SPM614, green) and Phalloidin (red).

Immunofluorescent staining of PFA-fixed human MCF7 cells with anti-Nucleolin antibody (clone SPM614, green) and Phalloidin (red).

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-Nucleolin antibody (clone SPM614).

Flow cytometry testing of PFA fixed human K562 cells with anti-Nucleolin antibody (clone SPM614); Red = isotype control, Blue = anti-Nucleolin antibody.