NSUN6 Antibody

• Catalog Number: orb1256530
• Category: Antibodies
• Description: NSUN6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-469 of human NSUN6 (NP_872349.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 52kDa
• Target: NSUN6
• UniProt ID: Q8TEA1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: NSUN6, 4933414E04Rik, FLJ23743, NOPD1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using NSUN6 antibody (orb1256530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 1 ug NSUN6 antibody (orb1256530). Western blot was performed from the immunoprecipitate using NSUN6 antibody (orb1256530) at a dilution of 1:1000.