NOTCH3 antibody

• Catalog Number: orb19112
• Category: Antibodies
• Description: Goat polyclonal antibody to NOTCH3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:16000, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 244
• Target: NOTCH3
• Entrez: 4854
• Protein Sequence: QLGPQPEVTPKRQ
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NOTCH3 antibody, anti Notch homolog 3 (Drosop
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.1. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Hippocampus using NOTCH3 antibody

Immunohistochemical staining of Human Placenta using NOTCH3 antibody

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using NOTCH3 antibody.