NOTCH3 Antibody

• Catalog Number: orb1263935
• Category: Antibodies
• Description: NOTCH3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Human
• Reactivity: Mouse
• Isotype: Rabbit Ig
• Immunogen: This NOTCH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2291-2321 amino acids from the C-terminal region of human NOTCH3.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 244 kDa
• Target: NOTCH3
• UniProt ID: Q9UM47
• NCBI: Q9UM47
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Neurogenic locus notch homolog protein 3, Notch 3,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western blot analysis in mouse NIH-3T3 cell line lysates (15 ug/lane).This demonstrates the NOTdetected the NOTCH3 protein (arrow).

Confocal immunofluorescent analysis of NOTCH3 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.