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Catalog Number | orb1672173 |
---|---|
Category | Antibodies |
Description | NOTCH1 Antibody |
Clonality | Recombinant |
Clone Number | E6 |
Tested applications | ELISA, IHC |
Reactivity | Human, Mouse |
Isotype | IgG lambda |
Immunogen | This antibody was selected against a fusion protein consisting of EGF domains 1–12 of murine Notch1 fused to a human Fc domain and binds to to the NRR of mouse Notch1. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | NOTCH1 |
UniProt ID | P46531, Q01705 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Tan1, Notch1, Mis6, N1, lin-12, NOTCH1, TAN1, hN1 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized r antibody (black line) or the rmeric version of E6 (orb1672173 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
Immunofluoresence staining of fixed HeLa cells with anti-Notch 1 antibody E6. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of E6 (orb1672173) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672173- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Canine, Guinea pig, Porcine, Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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