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Product Overview
Product Name NOTCH1 antibody
Catalog Number orb256723
Species/HostRabbit
ReactivityHuman, Mouse, Rat
ConjugationUnconjugated
Custom conjugations
Tested applicationsICC, IF, IHC-P, WB
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NOTCH1. The exact sequence is proprietary. Please contact us for the immunogen region. The peptide is available as orb257013.
Target NOTCH1. Recognizes endogenous levels of NOTCH1 protein.
Alternative Names
Product Properties
Form/Appearance Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Store at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity The antibody was purified by immunogen affinity chromatography.
Uniprot ID P46531 (Human); Q01705 (Mouse); Q07008 (Rat)
Entrez 4851
Product Description

Rabbit polyclonal antibody to NOTCH1, which is also known as AOVD. NOTCH1 is frequently found in the intracellular domain of the nucleus and is expressed in the basal keratinocytes and mesencephalic ventricular zone of the neural tube. It is overexpressed in the cerebrospinal fluid. This protein extensively functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.

Application Notes
Dilution Range WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Validation Images
Western blot analysis of HeLa (Lane1), NIH3T3 (Lane2) whole cell using NOTCH1 antibody
Western blot analysis of HeLa (Lane1), NIH3T3 (Lane2) whole cell using NOTCH1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human brain tissue using NOTCH1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human brain tissue using NOTCH1 antibody
Immunofluorescense analysis of HeLa cells lysates using NOTCH1 antibody
Immunofluorescense analysis of HeLa cells lysates using NOTCH1 antibody
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