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Catalog Number | orb1274424 |
---|---|
Category | Antibodies |
Description | NOTCH1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human NOTCH1 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 300kDa |
Target | NOTCH1 |
UniProt ID | P46531 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | NOTCH1, TAN1, hN1 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using NOTCH1 antibody (orb1274424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and NOTCH1 knockout (KO) HeLa cells, using NOTCH1 antibody (orb1274424) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spleen using NOTCH1 antibody (orb1274424) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NOTCH1 antibody (orb1274424) at dilution of 1:100. Blue: DAPI for nuclear staining.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Canine, Guinea pig, Porcine, Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P | |
Bovine, Canine, Equine, Gallus, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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