NKX2-1 Antibody

• Catalog Number: orb1252460
• Category: Antibodies
• Description: NKX2-1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NX2.1/690
• Tested applications: FC, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1, kappa
• Immunogen: Recombinant protein was used as the immunogen for the TTF1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the TTF1 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NKX2-1
• UniProt ID: P43699
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: NKX2-1 Antibody: BCH, BHC, NK-2, TEBP, TTF1, NKX2A
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the TTF1 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded rat lung stained with TTF1 antibody (NX2.1/690)

Formalin-fixed, paraffin-embedded human lung Adenocarcinoma stained with TTF1 antibody (NX2.1/690)

Formalin-fixed, paraffin-embedded human thyroid stained with TTF1 antibody (NX2.1/690)