NKX2-1 Antibody

• Catalog Number: orb1252069
• Category: Antibodies
• Description: NKX2-1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HBNK2-1
• Tested applications: FC, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1, kappa
• Immunogen: Recombinant protein was used as the immunogen for the TTF1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the TTF1 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NKX2-1
• UniProt ID: P43699
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: NKX2-1 Antibody: BCH, BHC, NK-2, TEBP, TTF1, NKX2A
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the TTF1 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human thyroid tissue with TTF1 antibody (clone HBNK2-1).

IHC testing of FFPE human lung tissue with TTF1 antibody (clone HBNK2-1).

IHC testing of FFPE rat lung tissue with TTF1 antibody (clone HBNK2-1).