You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1525420 |
---|---|
Category | Antibodies |
Description | NFIC Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | between 200 and 250 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | NFIC |
UniProt ID | P08651 |
NCBI | EAW69324.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | Nuclear factor 1/C; TGGCA-binding protein; Nuclear Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human NFIC by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NFIC antibody orb1525420 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Detection of human NFIC by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-NFIC Cat. No. orb1525420, left image used at a dilution of 1: 200 (1µg/ml) and Cat. No. orb1525418, right image used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Detection of mouse NFIC by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-NFIC Cat. No. orb1525420, left image used at a dilution of 1: 200 (1µg/ml) and Cat. No. orb1525418, right image used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
ELISA, FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating