NFIC Antibody

• Catalog Number: orb1525417
• Category: Antibodies
• Description: NFIC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 250 and 300
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NFIC
• UniProt ID: P08651
• NCBI: EAW69324.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Nuclear factor 1/C; TGGCA-binding protein; Nuclear
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NFIC by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NFIC antibody orb1525417 used for IP at 6 µg/mg lysate.

Detection of mouse NFIC by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-NFIC Cat. No. orb1525420, left image used at a dilution of 1: 200 (1µg/ml) and Cat. No. orb1525417, right image used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human NFIC by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-NFIC Cat. No. orb1525420, left image used at a dilution of 1: 200 (1µg/ml) and Cat. No. orb1525417, right image used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).