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Catalog Number | orb1525418 |
---|---|
Category | Antibodies |
Description | NFIC Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human |
Immunogen | between 250 and 300 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | NFIC |
UniProt ID | P08651 |
NCBI | EAW69324.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | Nuclear factor 1/C; TGGCA-binding protein; Nuclear Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human NFIC by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NFIC antibody orb1525418 used for IP at 6 µg/mg lysate.
Detection of mouse NFIC by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-NFIC Cat. No. orb1525420, left image used at a dilution of 1: 200 (1µg/ml) and Cat. No. orb1525418, right image used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human NFIC by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-NFIC Cat. No. orb1525420, left image used at a dilution of 1: 200 (1µg/ml) and Cat. No. orb1525418, right image used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
ELISA, FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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