NF-L antibody

• Catalog Number: orb1140387
• Category: Antibodies
• Description: Gallus polyclonal antibody to NF-L
• Species/Host: Gallus
• Clonality: Polyclonal
• Tested applications: ICC, WB
• Reactivity: Human, Mouse, Other, Rat
• Immunogen: To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, animals were boosted with recombinant mouse NF-L purified from bacteria.
• Concentration: 100ug/100ul
• Dilution range: The IgY solution can be used at dilutions of at least 1:2,500 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:5,000 or lower.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
• Note: For research use only
• Application notes: The IgY solution can be used at dilutions of at least 1:2,500 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:5,000 or lower.
• Expiration Date: 12 months from date of receipt.