NEFL Antibody

• Catalog Number: orb1261877
• Category: Antibodies
• Description: NEFL Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-543 of human NEFL (NP_006149.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 72kDa
• Target: NEFL
• UniProt ID: P07196
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: NEFL, 68 kDa neurofilament protein, CMT1F, CMT2E,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of MCF-7 cells, using NEFL antibody.

Immunohistochemistry of paraffin-embedded human breast cancer using NEFL antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using NEFL antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using NEFL antibody at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using NEFL antibody. Blue: DAPI for nuclear staining.