NeuN Rabbit Polyclonal Antibody (FITC)

SKU: orb187808

Description

NeuN Rabbit Polyclonal Antibody (FITC)

Images & Validation

• Tested Applications: FC, ICC, IF
• Dilution range: Flow-Cyt=1ug/test, IF=1:100-500, ICC/IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Equine, Insect, Mouse, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human FOX3 (101-200/312aa)
• Target: RBFOX3
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

RFOX3_HUMAN; RNA binding protein fox-1 homolog 3; RBFOX3; Fox-1 homolog C; Neuronal nuclei antigen(NeuN antigen); FOX3; FOX-3; HRNBP3;

Blank control: A549. Primary Antibody (green line): Rabbit Anti-NeuN antibody (orb187808), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

RBFOX3