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Catalog Number | orb547988 |
---|---|
Category | Antibodies |
Description | Nck NCK1 Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | AFEG-14 |
Tested applications | FC, ICC, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthesized peptide derived from human Nck Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Dilution range | WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
UniProt ID | P16333 |
Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Nck expression in HeLa cell lysate.
ICC, IHC-Fr, IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, IHC-P | |
Human, Monkey | |
Mouse | |
Monoclonal | |
Unconjugated |
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