NCK1 antibody

• Catalog Number: orb179922
• Category: Antibodies
• Description: Mouse monoclonal antibody to NCK1.
• Clonality: Monoclonal
• Clone Number: EM-06
• Tested applications: ICC, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant second SH3 domain of human Nck1 fused to GST
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 2 μg/ml; positive control: Jurkat cell lysate. Immunocytochemistry: Recommended dilution: 2 μg/ml; positive control: human NCK1-transfected COS-7 cells.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: NCK1
• Entrez: 4690
• UniProt ID: P16333
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-NCK-1 antibody, anti-NCKalpha antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 2 μg/ml; positive control: Jurkat cell lysate. Immunocytochemistry: Recommended dilution: 2 μg/ml; positive control: human NCK1-transfected COS-7 cells.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of NCK1 in whole cell lysate of mouse lymph node lymphocytes using the antibody EM-06.