NCDN Antibody

• Catalog Number: orb1247214
• Category: Antibodies
• Description: NCDN Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HYLLQVGSEKQKEP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and U251 (calculated MW of 77.2kDa according to NP_001014841.1). An additional band of unknown identity was also observed in U251 lysates at 55kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Mouse Kidney and Brain and NIH3T3 cell line for this particular batch.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NCDN
• UniProt ID: Q9UBB6
• NCBI: NP_055099.1, NP_001014841.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NCDN, neurochondrin , KIAA0607
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and U251 (calculated MW of 77.2kDa according to NP_001014841.1). An additional band of unknown identity was also observed in U251 lysates at 55kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Mouse Kidney and Brain and NIH3T3 cell line for this particular batch.
• Expiration Date: 12 months from date of receipt.

orb1247214 (0.3 ug/ml) staining of HepG2 (A) and (1 ug/ml) U251 cell lysate (B) + peptide (C) (35 ug protein in RIPA buffer). Detected by chemiluminescence.