NCDN antibody

• Catalog Number: orb19396
• Category: Antibodies
• Description: Goat polyclonal antibody to NCDN
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 78.9; 77.2
• Target: Neurochondrin
• Entrez: 23154
• Protein Sequence: HYLLQVGSEKQKEP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NCDN antibody, anti neurochondrin antibody, a
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 100kDa and140kDa in Human Brain (Cerebellum) lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.9kDa according to NP_055099.1 and 77.2kDa according to NP_001014841.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

WB analysis of HepG2 (A), U251 cell lysate (B) using NCDN antibody