N Cadherin antibody

• Catalog Number: orb705540
• Category: Antibodies
• Description: Mouse monoclonal antibody to N Cadherin
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human CD325. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:300
• Form/Appearance: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CDH2
• Entrez: 1000
• UniProt ID: P19022
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Alternative names: Anti-CDHN antibody, anti-NCAD antibody, anti-Cadhe
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD325 expression in HepG2 (A), Hela (B), A431 (C) whole cell lysates. (Predicted band size: 99 kD; Observed band size: 110 kD)

Immunohistochemical analysis of CD325 staining in human fallopian tube formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of CD325 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.