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Catalog Number | orb1294388 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to N-Cadherin |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / Extracellular domain corresponding to Human N-cadherin |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:200-1:300 Flow Cytometry 1:50-1:100 |
Conjugation | Unconjugated |
Target | N-cadherin (Extracellular) |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
N cadherin antibody at 1/1000 dilution, Lysates at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294388 overnight at 4°C. E-cadherin antibody (orb1294381).
Cell: H1299; Phalloidin: 1-300; N-cadherin (orb1294388): 1-200; Anti-rabbit 488: 1-300; NikonEpi-fluorescence (A-ken) 60x.
Immunohistochemical analysis of paraffin embedded Human testis cancer tissue labeling N-cadherin with orb1294388 at 1/100.
Immunohistochemical analysis of paraffin embedded Human lymph node cancer tissue labeling N-cadherin with orb1294388 at 1/100.
Product: N-cadherin antibody; Catalog: orb1294388; Cell: A549; N-cadherin (orb1294388): 1-100; Anti-rabbit 488: 1-1000.
Immunohistochemical analysis of paraffin embedded Human breast and kidney cancer tissue labeling N-cadherin with orb1294388 at 1/100.
FACS, IF, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, WB | |
Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep | |
Goat | |
Polyclonal | |
Unconjugated |
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