MyD88 Antibody

• Catalog Number: orb215902
• Category: Antibodies
• Description: MyD88 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MyD88 recombinant protein (Position: A44-F264). Human MyD88 shares 84% and 83% amino acid (aa) sequences identity with mouse and rat MyD88, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells ELISA (Cap), 1-5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 33233 MW
• UniProt ID: P35354
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Myeloid differentiation primary response protein M
• Note: For research use only
• Application notes: WB: The detection limit for MyD88 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-MYD88 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of MYD88 using anti-MYD88 antibody.Lane 1:Recombinant Human MYD88 Protein 0.5ng.

WB analysis of MYD88 using anti-MYD88 antibody.Lane 1:Rat Cardiac Muscle tissue;2:HELA cell;3:MCF cell;4:HEPG2 cell;5:JURKAT cell;6:RAJI Cell.

IF analysis of MYD88 using anti-MYD88 antibody.MYD88 was detected in paraffin-embedded section of human colon cancar tissues.

IF analysis of MYD88 using anti-MYD88 antibody.MYD88 was detected in paraffin-embedded section of human colon cancar tissues.

IF analysis of MYD88 using anti-MYD88 antibody.MYD88 was detected in paraffin-embedded section of human tonsil tissues.

IF analysis of MYD88 using anti-MYD88 antibody.MYD88 was detected in paraffin-embedded section of human mammary cancar tissues.

IF analysis of MYD88 using anti-MYD88 antibody.MYD88 was detected in paraffin-embedded section of human mammary cancar tissues.

IF analysis of MYD88 using anti-MYD88 antibody.MYD88 was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of MYD88 using anti-MYD88 antibody.MYD88 was detected in paraffin-embedded section of rat lung tissue.

IHC analysis of MYD88 using anti-MYD88 antibody.MYD88 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of MYD88 using anti-MYD88 antibody.MYD88 was detected in paraffin-embedded section of mouse spleen tissue.