MYC Antibody

• Catalog Number: orb1672344
• Category: Antibodies
• Description: MYC Antibody
• Clonality: Recombinant
• Clone Number: 9E10
• Tested applications: IF, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: MYC
• UniProt ID: P01106
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Proto-oncogene c-Myc, Transcription factor p64, Cl
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Left Panel: orb1672344 (2 ug/ml) staining of HEK293 spiked (A) and unspiked (B) with 5 ng multitag protein (35 ug protein in RIPA buffer). Lane C contains 25 ng purified multitag protein as positive control. Right Panel: .0 (1 ug/ml) staining of Multitag protein at 25 ng (A) - 10 ng (B) and 5 ng (C) amounts. For both - primary incubation was 1 hour. Detected by chemiluminescence.

ELISA of anti-epitope tag antibodies on multi-tag protein Plate coated with 5 ug/ml of Multitag protein (Pr00106-Antibody). Primary antibody loaded at 1000 ng/ml in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated goat anti-mouse (ec-0300-0108P).